Roseboro J A, Buchanan P, Norman A, Stern R
Phys Med Biol. 1978 Mar;23(2):324-31. doi: 10.1088/0031-9155/23/2/011.
Human peripheral blood and HeLa cells were irradiated in vitro at the ultrasonic frequency of 65 kHz. The whole blood and HeLa cell suspensions were exposed to continuous and pulsed ultrasonic power levels of 0.12, 0.16, 0.72, 1.12 and 2.24 W for a period of one minute. The method of ultrasonic irradiation was carried out with the whole blood or HeLa cell suspensions coupled directly to a cylindrical transducer while heating of the cell suspensions in excess of 41 degrees c was avoided. Irradiated and unirradiated peripheral blood lymphocyte chromosome cultures were prepared and scored for selected numerical and morphological abberrations. There was no significant difference in the frequency of chromosomal aberrations between irradiated and unirradiated cells. The fraction of cells S surviving after an exposure to an ultrasound dose of D can be represented by S = exp (-D/Do). Further, Do is shown to depend on the time after exposure at which survival is assayed.
人外周血和HeLa细胞在体外以65千赫的超声频率进行辐照。全血和HeLa细胞悬液分别暴露于0.12、0.16、0.72、1.12和2.24瓦的连续和脉冲超声功率水平下一分钟。超声辐照方法是将全血或HeLa细胞悬液直接与圆柱形换能器耦合，同时避免细胞悬液温度超过41摄氏度。制备辐照和未辐照的外周血淋巴细胞染色体培养物，并对选定的数量和形态异常进行评分。辐照细胞和未辐照细胞之间的染色体畸变频率没有显著差异。暴露于超声剂量D后存活的细胞分数S可以表示为S = exp (-D/Do)。此外，Do显示取决于暴露后测定存活情况的时间。
