Ellwart J W, Brettel H, Kober L O
Gesellschaft für Strahlen- und Umweltforschung, München.
Ultrasound Med Biol. 1988;14(1):43-50. doi: 10.1016/0301-5629(88)90162-7.
Cell membrane damage by ultrasound was studied in human nucleated cells in vitro at various concentrations. Suspensions of human blood cells, cells of a human leukemic cell line (Reh) and mixtures of nucleated cells with erythrocytes were exposed to continuous ultrasound of 782 kHz at a SPTA intensity of 15 W/cm2. The surviving nucleated cells without membrane damage were counted on the basis of exclusion of ethidium bromide using a flow cytometer. At high cell concentrations as present in whole blood, we observed no cell death, whereas below 5 X 10(7) cells/ml most of the granulocytes, stimulated lymphocytes and Reh cells were damaged. The concentration threshold below which cells were damaged seemed not to depend on the size of the cells, rather on the concentration of particles in the suspension.
在体外，研究了不同浓度下超声对人有核细胞细胞膜的损伤情况。将人血细胞悬液、人白血病细胞系（Reh）细胞以及有核细胞与红细胞的混合物，暴露于频率为782kHz、空间峰值时间平均（SPTA）强度为15W/cm²的连续超声下。使用流式细胞仪，基于溴化乙锭排除法对无细胞膜损伤的存活有核细胞进行计数。在全血中存在的高细胞浓度下，我们未观察到细胞死亡，而在低于5×10⁷个细胞/ml时，大多数粒细胞、受刺激的淋巴细胞和Reh细胞受到损伤。细胞受损的浓度阈值似乎并不取决于细胞大小，而是取决于悬浮液中颗粒的浓度。
