Krasnovskaia I A
Biull Eksp Biol Med. 1984 Nov;98(11):518-20.
Male Wistar rats were injected with 1 microgram thyroliberin twice a day for 8 days. Vasopressinergic (VE) and oxytocinergic (OE) cells of the supraoptic (SON) and paraventricular nuclei (PVN) were detected immunohistochemically, their changes were estimated morphometrically. The blood level of the thyrostimulating hormone determined by radioimmunoassay was 220% of the control on the 6th day but declined by the 9th day. The thyroid hormone concentration was also diminished. The nucleoli of the VE and OE cells of the SON were reduced by the 9th day (by 74 and 82%, respectively; P less than 0.01). The nucleoli of the OE cells of the PVN were enlarged to 120% (P less than 0.01), hence production of the neurohormone by these cells was intensified. The VE cells of the PVN remained essentially unchanged. The data obtained suggest that disagreement between the PVN and SON cell reactions is caused by various reactions of the nerve centers conveying toward the PVN and SON. Moreover, the OE and VE cells of the PVN apparently differ in their input. The importance of the OE cells of the PVN for the thyrostimulating hormone level normalization is suggested.
将雄性Wistar大鼠每天注射1微克促甲状腺素，持续8天，每天注射两次。采用免疫组织化学方法检测视上核（SON）和室旁核（PVN）的加压素能（VE）和催产素能（OE）细胞，并通过形态计量学评估其变化。通过放射免疫测定法测定的促甲状腺激素血药浓度在第6天为对照的220%，但在第9天下降。甲状腺激素浓度也降低。到第9天，SON的VE和OE细胞的核仁减少（分别减少74%和82%；P<0.01）。PVN的OE细胞核仁增大至120%（P<0.01），因此这些细胞的神经激素分泌增强。PVN的VE细胞基本保持不变。所得数据表明，PVN和SON细胞反应的不一致是由向PVN和SON传递的神经中枢的各种反应引起的。此外，PVN的OE和VE细胞的输入显然不同。提示PVN的OE细胞对促甲状腺激素水平正常化具有重要意义。
