Krasnovskaia I A
Probl Endokrinol (Mosk). 1984 Mar-Apr;30(2):52-5.
Three experiments were performed on Wistar male rats. In experiment N1 rats were maintained at the temperature of +5 degrees C during two hours, resultant in intensity of the thyroliberin synthesis. In test N2 synthetic thyroliberin in a dose of 8 micrograms was injected to intact rats 30 minutes before decapitation to decrease the requirement in the intrinsic hormone synthesis. In test N3 thyroliberin was injected to rats over the cooling process to compensate partially the need in the intrinsic hormone synthesis. The functional condition of the hypothalamic supraoptic-, suprachiasmatic-, paraventricular-, ventromedial and arcuate nuclei (SON, SCN, PVN, VMN, AN) was evaluated by morphometry. SON and SCN remained unchanged under cooling. In both tests thyroliberin injections reduced the nucleoli sizes in the nuclear cells. The AN response was different from that of other centers, i. e. in experiment N1 the AN activity decreased; in tests N2 and N3 the effect of thyroliberin injected was not revealed. The PVN and VMN cell activation was noted in experiment N1, whereas the activity of the same nuclei was markedly lowered in test N2. An increase in the activity of these nuclei in experiment N3 was slightly reduced under exogenous thyroliberin injection. It is suggested that the PVN and VMN synergy plays an essential role in the organism response to the change in the thyroliberin synthesis.
对雄性Wistar大鼠进行了三项实验。在实验N1中，将大鼠在+5摄氏度的温度下维持两小时，导致促甲状腺素释放激素合成强度增加。在实验N2中，在断头前30分钟向完整大鼠注射8微克剂量的合成促甲状腺素释放激素，以降低对自身激素合成的需求。在实验N3中，在冷却过程中向大鼠注射促甲状腺素释放激素，以部分补偿对自身激素合成的需求。通过形态计量学评估下丘脑视上核、视交叉上核、室旁核、腹内侧核和弓状核（SON、SCN、PVN、VMN、AN）的功能状态。冷却条件下SON和SCN保持不变。在两项实验中，注射促甲状腺素释放激素均使核细胞中的核仁尺寸减小。AN的反应与其他中枢不同，即在实验N1中AN活性降低；在实验N2和N3中，未显示注射促甲状腺素释放激素的效果。在实验N1中观察到PVN和VMN细胞激活，而在实验N2中相同核的活性明显降低。在实验N3中，这些核的活性增加在注射外源性促甲状腺素释放激素后略有降低。提示PVN和VMN的协同作用在机体对促甲状腺素释放激素合成变化的反应中起重要作用。
