促性腺激素释放激素激动剂（布舍瑞林）对食用蛙（食用蛙）睾丸体外和体内睾酮生成的刺激作用。

Stimulatory effect of a GnRH agonist (buserelin) in in vitro and in vivo testosterone production by the frog (Rana esculenta) testis.

作者信息

Pierantoni R, Fasano S, Di Matteo L, Minucci S, Varriale B, Chieffi G

出版信息

Mol Cell Endocrinol. 1984 Dec;38(2-3):215-9. doi: 10.1016/0303-7207(84)90120-5.

Abstract

The summary testicular effects of an agonistic analogue of gonadotropin-releasing hormone (buserelin, GnRHa) have been studied in vitro and in vivo in the frog, Rana esculenta. During 3 h incubation GnRHa (8 X 10(-7) M) potentiated pituitary factors in stimulating testosterone production by minced testes in vitro. After 6 h of incubation 8 X 10(-7) M GnRHa stimulated maximal testosterone output. Testes of 10-day hypophysectomized animals did not show any GnRHa effect in vitro. In vivo, a direct effect of GnRHa on testicular testosterone production was demonstrated in hypophysectomized animals, although this effect was temperature-dependent, requiring the frog to be maintained at a high temperature (24 degrees C). No effect of GnRHa was detectable in frogs kept at a low temperature (4 degrees C).

摘要

已在体外和体内对促性腺激素释放激素（布舍瑞林，GnRHa）的一种激动剂类似物对食用蛙（Rana esculenta）睾丸的影响进行了研究。在3小时的孵育过程中，GnRHa（8×10⁻⁷M）增强了垂体因子在体外刺激切碎睾丸产生睾酮的作用。孵育6小时后，8×10⁻⁷M GnRHa刺激睾酮产生达到最大值。垂体切除10天的动物的睾丸在体外未显示出任何GnRHa效应。在体内，GnRHa对垂体切除动物睾丸睾酮产生有直接影响，尽管这种影响取决于温度，要求青蛙维持在高温（24℃）下。在低温（4℃）饲养的青蛙中未检测到GnRHa的影响。