Sone S, Mutsuura S, Ishii K, Shirahama T, Tsubura E
Gan. 1984 Oct;75(10):920-8.
When human alveolar macrophages (AM) obtained by lavage of the lungs of healthy donors were incubated in medium with or without lipopolysaccharide (LPS) they released a factor(s) with tumor cell killing activity. This tumor cytolytic and/or cytotoxic factor(s) (TCF) was assayed by measuring its effect in inhibiting target cell growth. TCF activity was not observed in the supernatant from cultures of LPS-treated hematopoietic malignant cell lines (monocytic leukemia, B-cell leukemia and T-cell leukemia cell lines). Human TCF was significantly cytotoxic to 13 of 15 solid-tumor cell lines tested and to 7 of 9 hematopoietic malignant cell lines, but not to two different normal, nontumorigenic cell lines. TCF-rich supernatants contained low levels of interferon (IFN) activity that were not significantly cytotoxic to A-375 melanoma cells. Human TCF and IFN-alpha or IFN-beta had additive cytotoxic effects. These data suggest that human TCF released by activated human AM may be of potential use in the treatment of malignant disseminated diseases.
当通过灌洗健康供体的肺部获得的人肺泡巨噬细胞（AM）在含有或不含有脂多糖（LPS）的培养基中孵育时，它们会释放出具有肿瘤细胞杀伤活性的一种或多种因子。这种肿瘤细胞溶解和/或细胞毒性因子（TCF）通过测量其对靶细胞生长的抑制作用来进行测定。在LPS处理的造血恶性细胞系（单核细胞白血病、B细胞白血病和T细胞白血病细胞系）培养物的上清液中未观察到TCF活性。人TCF对所测试的15种实体瘤细胞系中的13种以及9种造血恶性细胞系中的7种具有显著的细胞毒性，但对两种不同的正常非致瘤细胞系无细胞毒性。富含TCF的上清液中含有低水平的干扰素（IFN）活性，对A - 375黑色素瘤细胞无显著细胞毒性。人TCF与IFN -α或IFN -β具有相加的细胞毒性作用。这些数据表明，活化的人AM释放的人TCF可能在恶性播散性疾病的治疗中具有潜在用途。
