Okubo A, Sone S, Singh S M, Ogura T
Third Department of Internal Medicine, University of Tokushima School of Medicine.
Jpn J Cancer Res. 1990 Apr;81(4):403-9. doi: 10.1111/j.1349-7006.1990.tb02582.x.
The abilities of human alveolar macrophages (AM) obtained from healthy donors and patients with lung cancer to produce tumor necrosis factor (TNF) were compared with those of their blood monocytes after activation with lipopolysaccharide (LPS). TNF activity was assayed by measuring cytotoxicity against actinomycin D-treated L929 cells and TNF was determined quantitatively by sandwich enzyme-linked immunosorbent assay (ELISA) with polyclonal and monoclonal antibodies against TNF-alpha. Unstimulated AM from healthy donors released variable amounts of TNF spontaneously, whereas blood monocytes did not. When treated with LPS for 24 h, AM and monocytes produced TNF dose-dependently, but TNF production by AM was significantly more than that by blood monocytes. This TNF activity was inhibited completely by monoclonal anti-TNF-alpha antibody. Macrophages generated by in vitro maturation of monocytes induced by granulocyte-macrophage colony-stimulating factor (GM-CSF) produced more TNF than freshly isolated monocytes. No difference was found in the abilities of AM from healthy donors and patients with lung cancer to produce TNF after activation stimuli. These observations suggest that human AM may be important in in vivo antitumor defense of the lung through TNF-alpha production.
将健康供体和肺癌患者的人肺泡巨噬细胞（AM）与经脂多糖（LPS）激活后的血液单核细胞产生肿瘤坏死因子（TNF）的能力进行了比较。通过测量对放线菌素D处理的L929细胞的细胞毒性来测定TNF活性，并使用针对TNF-α的多克隆和单克隆抗体通过夹心酶联免疫吸附测定（ELISA）对TNF进行定量测定。来自健康供体的未刺激AM自发释放出不同量的TNF，而血液单核细胞则不会。用LPS处理24小时后，AM和单核细胞均剂量依赖性地产生TNF，但AM产生的TNF明显多于血液单核细胞。这种TNF活性被单克隆抗TNF-α抗体完全抑制。由粒细胞-巨噬细胞集落刺激因子（GM-CSF）诱导的单核细胞体外成熟产生的巨噬细胞比新鲜分离的单核细胞产生更多的TNF。在激活刺激后，健康供体和肺癌患者的AM产生TNF的能力未发现差异。这些观察结果表明，人AM可能通过产生TNF-α在肺的体内抗肿瘤防御中起重要作用。
