Fei P F, Yue B Y, Tso M O
Graefes Arch Clin Exp Ophthalmol. 1984;222(1):1-8. doi: 10.1007/BF02133769.
The effect of chondroitin sulfate on the metabolism of trabecular meshwork was studied by repeatedly injecting a chondroitin sulfate solution into the anterior chamber of cat (experimental) eyes for 28-29 weeks. The aqueous humor of the opposite (control) eyes were similarly replaced with a phosphate-buffered saline-glucose solution. The intraocular pressure of the experimental eyes was increased 4-7 mm Hg above that of the control eyes for a prolonged period of time. In addition, collagen fibers, elastic fibers, and basement membrane-like material were moderately increased in the trabecular meshwork of experimental eyes. Three forms of thickened basement membranes of endothelial cells were observed. The compact multilaminated basement membrane was formed concentrically around some of the trabecular beams. The discontinuous, thin basement membrane was seen among bundles of coarse collagen in association with long spacing collagen fibers. Also, fine filaments were noted streaming from the plasma membrane and basement membrane of endothelial cells of angular aqueous veins. Chondroitinase ABC was able to remove ground substance in the elastic fiber of the trabecular meshwork. Biochemically, the chamber angle tissues from these eyes showed a higher synthetic activity of glycosaminoglycans and proteins. Chondroitin sulfate appears to have a direct effect on trabecular meshwork and can influence the physiologic function of these cells.
通过向猫（实验）眼的前房反复注射硫酸软骨素溶液28 - 29周，研究了硫酸软骨素对小梁网代谢的影响。对侧（对照）眼的房水则用磷酸盐缓冲盐水 - 葡萄糖溶液进行类似替换。实验眼的眼压在较长时间内比对照眼高出4 - 7毫米汞柱。此外，实验眼小梁网中的胶原纤维、弹性纤维和基底膜样物质适度增加。观察到内皮细胞的三种增厚基底膜形式。致密的多层基底膜围绕一些小梁束同心形成。在粗大胶原束与长间距胶原纤维相关的区域可见不连续的薄基底膜。此外，还注意到有细丝从角巩膜静脉内皮细胞的质膜和基底膜流出。硫酸软骨素酶ABC能够去除小梁网弹性纤维中的基质。从生化角度来看，这些眼睛的房角组织显示出糖胺聚糖和蛋白质的合成活性更高。硫酸软骨素似乎对小梁网有直接作用，并能影响这些细胞的生理功能。
