Gunning P, Mohun T, Ng S Y, Ponte P, Kedes L
J Mol Evol. 1984;20(3-4):202-14. doi: 10.1007/BF02104727.
The complete 3' untranslated region (3'UTR) sequence of the human skeletal-actin gene has been compared with the corresponding regions of the rat and chicken skeletal-actin genes. This comparison reveals that the skeletal-actin 3'UTR is composed of conserved and nonconserved segments. By using genomic Southern transfer blots and thermal stability (Tm) measurements, we found that the cardiac-actin gene 3'UTR also consists of conserved and nonconserved segments. Comparison of human and Xenopus laevis cardiac-actin mRNA sequences confirms the presence of a region of high similarity in the 3'UTR. We conclude that subsegments of the 3'UTRs of both skeletal- and cardiac-actin genes of birds and mammals are under considerable selective pressure. This suggests that these conserved sequences may have functional roles in actin-gene expression or regulation, and that these roles might be different for each actin isoform.
已将人类骨骼肌肌动蛋白基因完整的3'非翻译区（3'UTR）序列与大鼠和鸡骨骼肌肌动蛋白基因的相应区域进行了比较。该比较表明，骨骼肌肌动蛋白3'UTR由保守和非保守区段组成。通过使用基因组Southern转移印迹和热稳定性（Tm）测量，我们发现心肌肌动蛋白基因3'UTR也由保守和非保守区段组成。人类和非洲爪蟾心肌肌动蛋白mRNA序列的比较证实了3'UTR中存在高度相似的区域。我们得出结论，鸟类和哺乳动物的骨骼肌和心肌肌动蛋白基因的3'UTR亚片段都受到相当大的选择压力。这表明这些保守序列可能在肌动蛋白基因表达或调控中具有功能作用，并且这些作用可能因每种肌动蛋白同工型而异。
