抗黄曲霉毒素M1单克隆抗体的制备与鉴定
Production and characterization of monoclonal antibodies against aflatoxin M1.
作者信息
Woychik N A, Hinsdill R D, Chu F S
出版信息
Appl Environ Microbiol. 1984 Dec;48(6):1096-9. doi: 10.1128/aem.48.6.1096-1099.1984.
Abstract
By using an indirect enzyme-linked immunosorbent assay, four monoclonal antibodies were selected after fusion of mouse P3-NS1-Ag4-1 myeloma cells with spleen cells isolated from BALB/c mice that had been immunized with aflatoxin M1 (AFM1) conjugated to bovine serum albumin. Two of these antibodies were found to be specific for AFM1 and were designated AMW-1 and AMW-4. The specificities of AMW-1, which had higher affinity to AFM1, were determined by a competitive direct enzyme-linked immunosorbent assay with peroxidase-AFM1 as the marker. The relative cross-reactivity of each toxin (relative to AFM1) with AMW-1, as determined by the amount of aflatoxin necessary to cause 50% inhibition of enzyme activity, was 12, greater than 40, 12, and greater than 40 for B1, B2, G1, and G2, respectively.
摘要
通过使用间接酶联免疫吸附测定法，将小鼠P3-NS1-Ag4-1骨髓瘤细胞与从用与牛血清白蛋白偶联的黄曲霉毒素M1（AFM1）免疫的BALB/c小鼠分离的脾细胞融合后，筛选出了四种单克隆抗体。发现其中两种抗体对AFM1具有特异性，分别命名为AMW-1和AMW-4。以过氧化物酶-AFM1为标记，通过竞争性直接酶联免疫吸附测定法确定了对AFM1具有较高亲和力的AMW-1的特异性。根据导致50%酶活性抑制所需的黄曲霉毒素量确定，B1、B2、G1和G2与AMW-1的每种毒素的相对交叉反应性（相对于AFM1）分别为12、大于40、12和大于40。
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