黄曲霉毒素B2a抗血清的制备与特性分析
Production and characterization of aflatoxin B2a antiserum.
作者信息
Gaur P K, Lau H P, Pestka J J, Chu F S
出版信息
Appl Environ Microbiol. 1981 Feb;41(2):478-82. doi: 10.1128/aem.41.2.478-482.1981.
The specificity and sensitivity of antiserum elicited from rabbits against aflatoxin B2a-bovine serum albumin conjugates were characterized with a radioimmunoassay (RIA) and an enzyme-linked immunosorbent assay (ELISA). Aflatoxin B1 was first converted to aflatoxin B2a and then conjugated to bovine serum albumin and horseradish peroxidase by a reductive alkylation method. The antiserum was developed in New Zealand white rabbits by multiple-site injection with the aflatoxin B2a-bovine serum albumin conjugate. Antibody titers were determined by both RIA and ELISA. Competitive RIAs with various aflatoxin analogs indicated that the antiserum was most reactive with aflatoxin B1 and slightly cross-reactive with aflatoxins B2a, B2, and M1. Competitive ELISAs showed the antiserum to be equally specific for aflatoxins B2a and B12 and less reactive with aflatoxins B2 and M1. The relative sensitivities of RIA and ELISA for aflatoxin B1 quantitation were 100 and 10 pg per assay, respectively.
通过放射免疫分析（RIA）和酶联免疫吸附测定（ELISA）对兔抗黄曲霉毒素B2a - 牛血清白蛋白偶联物产生的抗血清的特异性和敏感性进行了表征。黄曲霉毒素B1首先转化为黄曲霉毒素B2a，然后通过还原烷基化方法与牛血清白蛋白和辣根过氧化物酶偶联。通过用黄曲霉毒素B2a - 牛血清白蛋白偶联物进行多点注射，在新西兰白兔中制备抗血清。通过RIA和ELISA测定抗体滴度。与各种黄曲霉毒素类似物的竞争性RIA表明，该抗血清与黄曲霉毒素B1反应性最强，与黄曲霉毒素B2a、B2和M1有轻微交叉反应。竞争性ELISA显示该抗血清对黄曲霉毒素B2a和B1同样具有特异性，而与黄曲霉毒素B2和M1的反应性较低。RIA和ELISA对黄曲霉毒素B1定量的相对灵敏度分别为每次测定100 pg和10 pg。
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