Whitney J B
Biochem Genet. 1984 Oct;22(9-10):913-22. doi: 10.1007/BF00499482.
A unique electrophoretic form of carbonic anhydrase is characteristic of some laboratory-maintained mice of the wild mouse species Mus spretus. This isozyme has been characterized by cellulose acetate electrophoresis and by isoelectric focusing. It is proposed that this isozyme be called CAR-2C and that its encoding allele be designated Car-2c. Fertile hybrids of Mus spretus and C57BL/6J (Car-2a) show both CAR-2A and CAR-2C bands of approximately equal intensity. The CAR-2C isozyme is readily identified by electrophoresis on 75-mm cellulose acetate strips because it migrates significantly faster than the isozymes of inbred mice, the CAR-2A and CAR-2B that do not separate from one another under standard conditions. Isoelectric focusing cleanly resolves all three of these CAR-2 forms. Mus hortulanus, although closely related to Mus spretus in other biochemical-genetic characteristics, has a CAR-2-homologous isozyme that is distinctly different from the CAR-2C of Mus spretus and from the isozymes of the common inbred strains.
一种独特的碳酸酐酶电泳形式是野生小家鼠（Mus spretus）的一些实验室饲养小鼠所特有的。这种同工酶已通过醋酸纤维素电泳和等电聚焦进行了表征。建议将这种同工酶称为CAR - 2C，并将其编码等位基因指定为Car - 2c。小家鼠（Mus spretus）和C57BL/6J（Car - 2a）的可育杂种显示出强度大致相等的CAR - 2A和CAR - 2C条带。通过在75毫米醋酸纤维素条上进行电泳，可以很容易地鉴定出CAR - 2C同工酶，因为它的迁移速度明显快于近交系小鼠的同工酶CAR - 2A和CAR - 2B，在标准条件下它们彼此无法分离。等电聚焦可以清晰地分辨出所有这三种CAR - 2形式。短尾小家鼠（Mus hortulanus）虽然在其他生化遗传特征上与小家鼠（Mus spretus）密切相关，但其具有一种与CAR - 2同源的同工酶，该同工酶与小家鼠（Mus spretus）的CAR - 2C以及常见近交系的同工酶明显不同。
