Waksman G, Hamel E, Bouboutou R, Besselièvre R, Fournié-Zaluski M C, Roques B P
C R Acad Sci III. 1984;299(14):613-5.
The first visualization of enkephalinase (neutral metalloendopeptidase, E.C.3.4.24.11) in rat brain was obtained by autoradiography, using a new tritiated inhibitor: [3H]N-[( R,S )3-(N-hydroxy) carboxamido-2-benzyl propanoyl]glycine (3H-HCBP-Gly). The preliminary analysis of sections clearly showed a discrete localization of enkephalinase in enkephalin enriched regions, such as caudate nucleus, putamen, globus pallidus, and substantia nigra. Moreover 3H-HCBP-Gly binding also occurred in choroid plexus and spinal cord.
通过放射自显影技术，使用一种新的氚标记抑制剂：[3H]N-[(R,S)3-(N-羟基)羧酰胺基-2-苄基丙酰基]甘氨酸（3H-HCBP-Gly），首次在大鼠脑中观察到脑啡肽酶（中性金属内肽酶，E.C.3.4.24.11）。切片的初步分析清楚地显示脑啡肽酶在富含脑啡肽的区域有离散定位，如尾状核、壳核、苍白球和黑质。此外，3H-HCBP-Gly结合也出现在脉络丛和脊髓中。
