Gaudoux F, Boileau G, Crine P
Department of Biochemistry, Faculty of Medicine, University of Montreal, Quebec, Canada.
J Neurosci Res. 1993 Mar 1;34(4):426-33. doi: 10.1002/jnr.490340407.
The distribution of the messenger RNA (mRNA) coding for neprilysin (EC 3.4.24.11) has been studied by in situ hybridization in the adult rat brain. A markedly heterogenous distribution among various brain regions was found. A strong signal was observed in the glomerular layer of the olfactory bulb, the olfactory tubercle, the caudate putamen, the habenular, anterior pretectal, interpeduncular, red, dorso tegmental, pontine, and vestibular nuclei, the mammillary bodies, the Purkinje cells, and the choroid plexus of the fourth ventricle. A large number of areas such as the cortex, the dentate gyrus, the hippocampus, the medial terminal nucleus of the accessory tract, the accumbens and the arcuate nuclei, the superior and inferior colliculi, and a few regions in the thalamus at the mesencephalic level exhibited a moderate or low signal of hybridization. The majority of these regions are also known to contain the neprilysin protein. On the other hand, the globus pallidus, the substantia nigra, and the central gray matter, which show a high or a moderate amount of neprilysin, did not contain any neprilysin mRNA. Comparison of the regional distribution of neprilysin mRNA with that of its translation product provides insight into neprilysin neuronal pathways in the central nervous system.
通过原位杂交技术，对成年大鼠脑中编码中性内肽酶（EC 3.4.24.11）的信使核糖核酸（mRNA）分布进行了研究。结果发现，不同脑区的分布明显不均一。在嗅球的肾小球层、嗅结节、尾状壳核、缰核、前顶盖前区、脚间核、红核、背侧被盖核、脑桥核、前庭核、乳头体、浦肯野细胞以及第四脑室脉络丛中观察到强信号。大量区域，如皮质、齿状回、海马体、副束内侧终核、伏隔核和弓状核、上丘和下丘以及中脑水平丘脑的一些区域，呈现出中度或低度杂交信号。这些区域中的大多数也已知含有中性内肽酶蛋白。另一方面，苍白球、黑质和中央灰质，虽然显示出高含量或中等含量的中性内肽酶，但却不含任何中性内肽酶mRNA。比较中性内肽酶mRNA与其翻译产物的区域分布，有助于深入了解中枢神经系统中中性内肽酶的神经元通路。
