Anastassiades T, Irwin D, Woods A, Robertson W
Comp Biochem Physiol B. 1984;79(4):623-31. doi: 10.1016/0305-0491(84)90376-6.
Human and bovine bone matrices were extracted with salt solutions of different composition and the extracts tested for stimulation of incorporation of radioactivity from [3H]glucosamine and [35S]SO4 into the hyaluronic acid and chondroitin sulfate of the cell pellet, the cell surface and the medium fractions of human synovial cells in culture. Stimulatory activity was extracted with a solution of 0.3 M EDTA in 2.5 M NaCl from bovine but not human bone. Subsequent extraction of the residues with 4 M guanidinium hydrochloride yielded activity from both matrices. A major stimulation of incorporation of radioactivity was observed in the cell surface fractions. Human synovial cells constitute a more sensitive assay system for the stimulatory activity than rabbit synovial cells.
用不同成分的盐溶液提取人骨和牛骨基质，并对提取物进行检测，以观察其对培养的人滑膜细胞细胞沉淀、细胞表面及培养基组分中透明质酸和硫酸软骨素从[3H]葡萄糖胺和[35S]SO4掺入放射性的刺激作用。用0.3M EDTA溶于2.5M NaCl的溶液可从牛骨而非人骨中提取出刺激活性。随后用4M盐酸胍提取残渣，两种基质均产生活性。在细胞表面组分中观察到放射性掺入有显著刺激作用。与人滑膜细胞相比，人滑膜细胞对刺激活性构成更敏感的检测系统。
