Anastassiades T P, Starkey P
Cell Differ. 1983 Dec;13(4):341-8. doi: 10.1016/0045-6039(83)90044-1.
Confluent cultures of rat muscle fibroblastic cells respond by increased glycosaminoglycan (GAG) synthesis when cultured in medium containing a solubilized bone matrix fraction (SBM) at a concentration of 100 micrograms/ml. The metabolism of the GAG associated with the cell pellet, the cell surface and the tissue culture medium fractions was studied, in the presence and absence of SBM, by measuring the incorporation of radioactivity from [3H]glucosamine and [35S]SO4 into the isolated GAG. Net synthesis of hyaluronic acid and of chondroitin sulfate in the medium fraction increased more rapidly in cultures containing SBM compared to controls, and the accumulation of labelled GAG in the medium of the treated cultures was approximately linear with respect to the length of incubation. The addition of SBM also resulted in increased incorporation of 3H and of 35S into the GAG of the cell surface and cell pellet fractions. In these fractions, stimulation of incorporation of radioactivity occurred in two waves: an early, relatively minor increase and a later relatively major increase. The relatively major stimulation of radioactivity into the GAG of the cell surface fraction occurred between 24 and 48 h and was independent of any apparent effect of serum.
当在含有浓度为100微克/毫升的溶解骨基质组分（SBM）的培养基中培养时，大鼠肌肉成纤维细胞的汇合培养物会通过增加糖胺聚糖（GAG）合成来做出反应。通过测量[3H]葡萄糖胺和[35S]SO4中的放射性掺入到分离的GAG中，研究了在有和没有SBM的情况下，与细胞沉淀、细胞表面和组织培养基组分相关的GAG的代谢。与对照相比，在含有SBM的培养物中，培养基组分中透明质酸和硫酸软骨素的净合成增加得更快，并且在处理过的培养物的培养基中标记GAG的积累相对于孵育时间近似呈线性。添加SBM还导致3H和35S掺入到细胞表面和细胞沉淀组分的GAG中的量增加。在这些组分中，放射性掺入的刺激以两个波出现：一个早期的、相对较小的增加和一个后期的、相对较大的增加。细胞表面组分的GAG中放射性的相对较大刺激发生在24至48小时之间，并且与血清的任何明显作用无关。
