Zipf T F, Bryant L D, Koskowich G N, MacGregor S E, Chin L, Johnson H
Cytometry. 1984 Nov;5(6):610-3. doi: 10.1002/cyto.990050609.
The pre-B phenotype of childhood acute lymphoblastic leukemia, in which the mu immunoglobulin molecule is expressed in the cytoplasm of the blast cells, has been shown to have independent prognostic significance. Patients with this phenotype of the disease tend to have a poorer outcome on contemporary treatment regimens than do those with the other B-precursor phenotypes. For these clinical studies, the detection of the pre-B phenotype has depended upon fluorescent-microscope based techniques. A flow-cytometry based technique for the detection of the cytoplasmic mu immunoglobulin molecule is presented here and the results compared in detail with those of fluorescent microscopy. The results show that the two techniques are equivalent. The flow cytometry method has the advantage of a standardized control, is less labor intensive, and is observer-independent.
儿童急性淋巴细胞白血病的前B表型，即原始细胞胞质中表达μ免疫球蛋白分子，已被证明具有独立的预后意义。患有这种疾病表型的患者在当代治疗方案下的预后往往比其他B前体细胞表型的患者更差。在这些临床研究中，前B表型的检测依赖于基于荧光显微镜的技术。本文介绍了一种基于流式细胞术检测胞质μ免疫球蛋白分子的技术，并将结果与荧光显微镜检测结果进行了详细比较。结果表明这两种技术等效。流式细胞术方法具有标准化对照的优势，劳动强度较小，且不依赖观察者。
