Nagy K, Nagy I
Mech Ageing Dev. 1984 Dec;28(2-3):171-6. doi: 10.1016/0047-6374(84)90017-4.
Properly prepared membrane proteins of brain synaptosomes of 2-, 12- and 24-month-old CFY female rats were filtrated on a Sepharose 2B gel. The molecular weight distribution showed an age-dependence: there was a clear shift toward the higher molecular weights in the adult and old rats. The observed alterations reflect an increased cross-linking of the proteins during aging due most probably to the OH free radical damage of the cell components. Centrophenoxine treatment for 2 months reversed this phenomenon in the old animals: the high molecular weight fractions decreased and the lower ones increased in the treated animals as compared to the old, untreated rats. The results support the membrane hypothesis of aging and contribute to a better understanding of the biological effects of centrophenoxine.
对2个月、12个月和24个月大的CFY雌性大鼠脑突触体的膜蛋白进行适当制备后，在琼脂糖2B凝胶上进行过滤。分子量分布显示出年龄依赖性：成年和老年大鼠中明显向更高分子量转移。观察到的变化反映出衰老过程中蛋白质交联增加，这很可能是由于细胞成分受到羟基自由基损伤所致。对老年动物进行2个月的盐酸甲氯芬酯治疗可逆转这一现象：与未治疗的老年大鼠相比，治疗组动物中高分子量部分减少，低分子量部分增加。这些结果支持衰老的膜假说，并有助于更好地理解盐酸甲氯芬酯的生物学效应。
