Enzymatic synthesis of anticodon-deleted and replaced Bacillus subtilis tRNAThr and their amino acid acceptor activity.

By some enzymatic and chemical procedures, one or two nucleotides in the anticodon region of Bacillus subtilis tRNAThr were deleted and a part of the anticodon sequence was replaced. The anticodon-deleted molecules retained amino acid acceptor activity although the activity was low. However, the anticodon-replaced molecule which has a lysine specific sequence (U-U-U) exhibited neither threonine acceptor activity nor lysine acceptor activity.