Nishikawa K, Uritani M, Miyazaki M, Takemura S
Nucleic Acids Symp Ser. 1984(15):125-8.
A variant of T. utilis tRNATyr containing a base substitution (psi----A) in the middle position of the anticodon has been constructed by enzymatic procedures in vitro. This variant is unique in that it can accept both tyrosine and phenylalanine. This tRNA was shown to be active in transferring both tyrosine and phenylalanine into polypeptides in a cell-free, poly (U)-directed translation system from yeast. This result gives further support to the adapter hypothesis since tyrosine, attached to the variant tRNATyr with an anticodon G-A-A, is incorporated into polypeptides in response to poly (U) message.
通过体外酶促方法构建了一种嗜热栖热菌酪氨酸转运核糖核酸（tRNATyr）变体，其反密码子中间位置存在碱基替换（假尿苷——腺嘌呤）。该变体的独特之处在于它既能接受酪氨酸，也能接受苯丙氨酸。在来自酵母的无细胞、聚尿苷酸（poly (U)）指导的翻译系统中，这种转运核糖核酸在将酪氨酸和苯丙氨酸都转移到多肽中时表现出活性。这一结果进一步支持了适配体假说，因为与反密码子G - A - A相连的酪氨酸会响应聚（U）信息而被掺入多肽中。
