Tcholakian R K, Berkowitz A S, Newaz S N
Steroids. 1984 Apr;43(4):457-67. doi: 10.1016/0039-128x(84)90019-9.
Sertoli cells from immature rats metabolized (3H) 5 alpha-androstane-3 alpha, 17 beta-diol to (3H) 5 alpha-androstane-3 alpha, 16 alpha, 17 beta-triol and (3H) 3 alpha-hydroxy-5 alpha-androstan-17-one. This is the first report of 16 alpha-hydroxylation of 5 alpha-reduced androgens in the testis. FSH significantly stimulated 16 alpha-hydroxylation while LH significantly decreased this activity. 3 alpha-Hydroxy-5 alpha-androstan-17-one was the major metabolite formed and its production was significantly increased in the presence of both LH and FSH, although FSH stimulation was significantly more than LH. The possible role of 16 alpha-hydroxylase in androgen metabolism by immature rat Sertoli cells is discussed.
未成熟大鼠的支持细胞将（3H）5α-雄甾烷-3α，17β-二醇代谢为（3H）5α-雄甾烷-3α，16α，17β-三醇和（3H）3α-羟基-5α-雄甾-17-酮。这是睾丸中5α-还原雄激素16α-羟基化的首次报道。促卵泡激素（FSH）显著刺激16α-羟基化，而促黄体生成素（LH）则显著降低该活性。3α-羟基-5α-雄甾-17-酮是形成的主要代谢产物，在同时存在LH和FSH的情况下其产量显著增加，尽管FSH的刺激作用明显大于LH。文中讨论了16α-羟化酶在未成熟大鼠支持细胞雄激素代谢中的可能作用。
