Martynov Iu V, Samsonova I M, Demina A A
Zh Mikrobiol Epidemiol Immunobiol. 1984 Oct(10):27-30.
The possibility of using, on principle, the reaction of radial hemolysis for the determination of antibodies to meningococci has been shown. The sensitivity and resolution of this method has been found to depend on the dose of the antigen used for the sensitization of erythrocytes, on the concentration of the erythrocyte suspension introduced into the gel and on the amount of complement. The optimum conditions for the reaction of optimum hemolysis, used for the determination of antibodies to serogroup A Neisseria meningitidis polysaccharide, have been established: the sensitizing dose of the antigen must be 50-100 micrograms/ml, the concentration of sensitized erythrocytes 25%, and the amount of complement 20-40 HU.
原则上，已证明可利用放射状溶血反应来测定抗脑膜炎球菌抗体。已发现该方法的灵敏度和分辨率取决于用于致敏红细胞的抗原剂量、引入凝胶中的红细胞悬液浓度以及补体的量。已确定用于测定A群脑膜炎奈瑟菌多糖抗体的最佳溶血反应的最佳条件：抗原的致敏剂量必须为50 - 100微克/毫升，致敏红细胞的浓度为25%，补体的量为20 - 40 HU。
