Eisenmann D R, Ashrafi S H, Zaki A E
Calcif Tissue Int. 1984 Sep;36(5):596-603. doi: 10.1007/BF02405373.
Energy dispersive X-ray microanalysis was applied to freeze-dried blocks of enamel organ tissue to determine levels of calcium in various cellular regions. The tissue blocks were dissected free from adjacent forming enamel following injection of cobalt or fluoride ions, both of which temporarily inhibit enamel mineralization. In all control and experimental specimens there was an increasing gradient of calcium from the stratum intermedium cells to the distal ends of the ameloblasts. Calcium levels were significantly reduced near the distal ends of the ameloblasts following cobalt or fluoride injection as compared with controls. It is suggested that evidence of an intercellular buildup of calcium near the distal ends of the ameloblast supports a controlling function of these cells. The changes in calcium levels are correlated with alterations in mineralization known to occur in the adjacent enamel of the model systems employed.
能量色散X射线微分析技术应用于牙釉质器官组织的冻干块，以确定不同细胞区域的钙含量。在注射钴离子或氟离子后，将组织块从相邻的正在形成的牙釉质中分离出来，这两种离子都会暂时抑制牙釉质矿化。在所有对照和实验样本中，从中间层细胞到成釉细胞远端，钙含量呈递增梯度。与对照组相比，注射钴或氟后，成釉细胞远端附近的钙含量显著降低。这表明，成釉细胞远端附近细胞间钙积累的证据支持了这些细胞的控制功能。钙含量的变化与所用模型系统相邻牙釉质中已知发生的矿化改变相关。
