发育中小鼠磨牙钙化基质和形成细胞的微探针分析

Microprobe analysis of calcifying matrices and formative cells in developing mouse molars.

作者信息

Engel M B

出版信息

Histochemistry. 1981;72(3):443-52. doi: 10.1007/BF00501786.

Abstract

The distribution of Calcium and Phosphorus and of Na, K, S and Cl was studied in the mineralizing matrices and strata of ameloblasts and odontoblasts in developing mouse molars (5-14 days). Sections cut in a cryostat were prepared by freeze-drying and examined in an SEM by the method of energy dispersive x-ray analysis. In enamel a gradient of mineralization was observed with respect to age and topography. Progesssive loss of sulfur was also demonstrated. Less striking mineralization gradients were found in dentin. Predentin accumulated Ca at a concentration about 2% that of dentin and the Ca/P ratio was lower than that for apatite. Significant concentrations of calcium were localized in ameloblast and odontoblast strata. The concentration increased five-fold in ameloblasts as the cells matured and enamel mineralization entered the final phases, levels in odontoblasts remained stable. With age in both cellular strata, potassium counts decreased. In maturing ameloblasts the concentrations of sodium and chloride rose.

摘要

对发育中小鼠磨牙（5 - 14天）成釉细胞和成牙本质细胞的矿化基质及层中的钙、磷以及钠、钾、硫和氯的分布进行了研究。通过冷冻干燥制备在低温恒温器中切割的切片，并采用能量色散X射线分析方法在扫描电子显微镜下进行检查。在牙釉质中，观察到矿化随年龄和地形的梯度变化。还证实了硫的逐渐流失。在牙本质中发现的矿化梯度不太明显。前期牙本质积累的钙浓度约为牙本质的2%，且钙磷比低于磷灰石。钙的显著浓度定位于成釉细胞层和成牙本质细胞层。随着细胞成熟且牙釉质矿化进入最后阶段，成釉细胞中的浓度增加了五倍，而成牙本质细胞中的水平保持稳定。随着两个细胞层年龄的增长，钾计数减少。在成熟的成釉细胞中，钠和氯的浓度升高。