Kominami E, Bando Y, Wakamatsu N, Katunuma N
J Biochem. 1984 Nov;96(5):1437-42. doi: 10.1093/oxfordjournals.jbchem.a134972.
The concentrations of two types of endogenous inhibitors of thiol proteinases were determined in soluble extracts of various rat tissues by means of a sensitive enzyme immunoassay method, which consisted of solid-phase immobilized anti-rat liver inhibitor or anti-rat epidermal inhibitor and antibodies labeled with horseradish peroxidase. The minimum detectable amounts of inhibitors from liver and epidermis were 30 pg and 3 pg/assay, respectively. The tissue distributions of the epidermal and liver-type inhibitors were found to differ. The liver-type inhibitor was found to be widely distributed in various tissues at levels of 76-420 ng/mg protein, whereas the epidermal-type inhibitor was found at high levels in the skin, tongue, esophagus, stomach, intestine, and vagina, but at quite low levels in other tissues tested.
采用一种灵敏的酶免疫测定法，测定了多种大鼠组织可溶性提取物中两种巯基蛋白酶内源性抑制剂的浓度。该方法包括固相固定的抗大鼠肝脏抑制剂或抗大鼠表皮抑制剂以及用辣根过氧化物酶标记的抗体。每次测定中，肝脏和表皮抑制剂的最低可检测量分别为30 pg和3 pg。发现表皮型和肝脏型抑制剂的组织分布不同。肝脏型抑制剂在各种组织中广泛分布，含量为76 - 420 ng/mg蛋白质，而表皮型抑制剂在皮肤、舌头、食道、胃、肠道和阴道中含量较高，但在其他测试组织中含量相当低。
