Rinne A, Järvinen M, Räsänen O
Acta Histochem. 1978;63(2):183-92. doi: 10.1016/S0065-1281(78)80024-5.
The occurence of the human and rat epidermal SH-protease inhibitors in various human and rat tissues was studied by double radial immunodiffusion against specific antisera to the inhibitors. An immunoreactive protein was found in the extracts prepared from human and rat epidermis and from eosophageal and vaginal squamous epithelia, and from rat pro-ventricular squamous epithelium. No immunoreactive protein was found in man or rat in any other of their tissues, studied by us. The results strongly suggest that a protein reminiscent of the human or rat epidermal SH-protease inhibitor is present in squamous epithelia but not in other tissues. The identity of the epidermal inhibitor and the immunoreactive protein in the other squamous epithelia was confirmed by immunodiffusion, immunoelectrophoresis and gel chromatography, and by immunoinhibition of the papain inhibiting activity of the human epidermal and oesophageal inhibitors by gammaglobulins separated from antiserum to the human epidermal inhibitor.
采用针对人及大鼠表皮 SH 蛋白酶抑制剂特异性抗血清的双向放射免疫扩散法，研究了人及大鼠表皮 SH 蛋白酶抑制剂在人及大鼠各种组织中的分布情况。在人及大鼠表皮、食管和阴道鳞状上皮以及大鼠前脑室鳞状上皮提取物中发现了一种免疫反应性蛋白。在我们所研究的人或大鼠的任何其他组织中均未发现免疫反应性蛋白。结果强烈表明，在鳞状上皮中存在一种类似于人或大鼠表皮 SH 蛋白酶抑制剂的蛋白，而在其他组织中则不存在。通过免疫扩散、免疫电泳和凝胶色谱法，以及用从抗人表皮抑制剂血清中分离的γ球蛋白对人表皮和食管抑制剂的木瓜蛋白酶抑制活性进行免疫抑制，证实了表皮抑制剂与其他鳞状上皮中免疫反应性蛋白的同一性。
