Hudman J F, Glenn A R
Arch Microbiol. 1984 Dec;140(2-3):252-6. doi: 10.1007/BF00454937.
Selenite uptake and incorporation in Selenomonas ruminantium was constitutive with an inducible component. It was distinct from sulphate or selenate transport, since sulphate and selenate did not inhibit uptake, nor could sulphate or selenate uptake be demonstrated. Selenite uptake had an apparent Km of 1.28 mM and a Vmax of 148 ng Se min-1 mg-1 protein. Uptake was sensitive to inhibition by 2,4-dinitrophenol (DNP), carbonyl cyanide m-chlorophenyl hydrazone (CCCP), azide, iodoacetic acid (IAA) and N-ethylmaleimide (NEM), but not chlorpromazine (CPZ), N,N'-dicyclohexyl-carbodiimide (DCCD), quinine, arsenate, or fluoride. Treatment of cells accumulating 75[Se]-Selenite with 2,4,DNP inhibited uptake, but did not cause efflux. Transport of selenite was inhibited by sulphite and nitrite, but not by nitrate, phosphate, sulphate of selenate. 75[Se]-Selenite was incorporated into selenocystine, selenoethionine, selenohomocysteine, and selenomethionine and was also reduced to red elemental selenium.
瘤胃月形单胞菌对亚硒酸盐的摄取和掺入是组成型的且带有一个可诱导成分。它与硫酸盐或硒酸盐的转运不同，因为硫酸盐和硒酸盐不会抑制摄取，也未证明存在硫酸盐或硒酸盐的摄取。亚硒酸盐摄取的表观 Km 为 1.28 mM，Vmax 为 148 ng Se min⁻¹ mg⁻¹ 蛋白质。摄取对 2,4 - 二硝基苯酚（DNP）、羰基氰 m - 氯苯腙（CCCP）、叠氮化物、碘乙酸（IAA）和 N - 乙基马来酰亚胺（NEM）的抑制敏感，但对氯丙嗪（CPZ）、N,N'-二环己基碳二亚胺（DCCD）、奎宁、砷酸盐或氟化物不敏感。用 2,4 - DNP 处理积累 75[Se] - 亚硒酸盐的细胞会抑制摄取，但不会导致外排。亚硒酸盐的转运受到亚硫酸盐和亚硝酸盐的抑制，但不受硝酸盐、磷酸盐、硫酸盐或硒酸盐的抑制。75[Se] - 亚硒酸盐被掺入到硒代胱氨酸、硒代乙硫氨酸、硒代高半胱氨酸和硒代甲硫氨酸中，并且还被还原为红色元素硒。
