氧气对酿酒酵母微粒体电子传递系统的影响。
Influence of oxygen on the microsomal electron transport system in Saccharomyces cerevisiae.
作者信息
Bertrand J C, Mattei G, Parra C, Giordani R, Gilewicz M
出版信息
Biochimie. 1984 Jul-Aug;66(7-8):583-8. doi: 10.1016/0300-9084(84)90155-x.
The influence of oxygen on the level of microsomal electron transport chain components has been studied during the growth of Saccharomyces cerevisiae. Enzyme activities and cytochrome content were assayed in microsomal fractions prepared from a protoplast lysate free from mitochondrial contamination. It was found that the cytochrome P-450 and cytochrome b5 content, to get her with the NADPH-cytochrome (P-450)-reductase and NADH-cytochrome (b5)-reductase activities, were increased in the cells as the pO2 of the medium was decreasing. At the same time an increase in the membrane surface of the endoplasmic reticulum can be observed.
在酿酒酵母生长过程中，研究了氧气对微粒体电子传递链成分水平的影响。从无线粒体污染的原生质体裂解物制备的微粒体组分中测定了酶活性和细胞色素含量。结果发现，随着培养基中pO2的降低，细胞中的细胞色素P-450和细胞色素b5含量以及NADPH-细胞色素（P-450）还原酶和NADH-细胞色素（b5）还原酶活性均增加。同时，可以观察到内质网膜表面增加。
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