Merritt J E, Dobson P R, Wojcikiewicz R J, Baird J G, Brown B L
Biosci Rep. 1984 Dec;4(12):995-1000. doi: 10.1007/BF01116691.
A possible role for Ca2+ and calmodulin in the action of growth-hormone-releasing factor (GHRF) was investigated. Low extracellular Ca2+ (less than 100 microM), methoxyverapamil, flunarizine, cinnarizine, and Co2+ decreased both basal and GHRF-stimulated growth-hormone secretion, but did not totally inhibit GHRF-stimulated secretion. A calmodulin antagonist, W7, abolished GHRF-stimulated GH secretion, with no effect on basal secretion. It is suggested that GHRF may act primarily by elevating cellular cyclic AMP, which may then modulate calcium mobilization or flux; the increased intracellular Ca2+ concentrations may then activate calmodulin.
研究了钙离子（Ca2+）和钙调蛋白在生长激素释放因子（GHRF）作用中的可能作用。细胞外低钙（低于100微摩尔）、甲氧维拉帕米、氟桂利嗪、桂利嗪和钴离子（Co2+）降低基础和GHRF刺激的生长激素分泌，但并未完全抑制GHRF刺激的分泌。一种钙调蛋白拮抗剂W7消除了GHRF刺激的生长激素分泌，对基础分泌无影响。提示GHRF可能主要通过提高细胞内环磷酸腺苷（cAMP）起作用，后者继而可能调节钙动员或钙通量；细胞内Ca2+浓度升高可能随后激活钙调蛋白。
