一种改进的酶联免疫测定法，用于检测和定量苏云金芽孢杆菌库尔斯塔克亚种和以色列亚种的杀虫伴孢晶体蛋白。
An improved enzyme-linked immunoassay for the detection and quantification of the entomocidal parasporal crystal proteins of Bacillus thuringiensis subsp. kurstaki and israelensis.
作者信息
Wie S I, Hammock B D, Gill S S, Grate E, Andrews R E, Faust R M, Bulla L A, Schaefer C H
出版信息
J Appl Bacteriol. 1984 Dec;57(3):447-54. doi: 10.1111/j.1365-2672.1984.tb01410.x.
An improved and simplified enzyme-linked immunosorbent assay (ELISA) was developed for the detection and quantification of parasporal crystalline toxins from Bacillus thuringiensis subsp. kurstaki. The improved procedure involved pretreatment of the polystyrene cuvettes with glutaraldehyde before antibody coating. A direct comparison of treated and untreated cuvettes is provided. ELISAs were then used for the analysis of the entomocidal crystalline proteins in commercial and experimental formulations of B. thuringiensis subspp. kurstaki and israelensis.
开发了一种改进和简化的酶联免疫吸附测定法（ELISA），用于检测和定量苏云金芽孢杆菌库尔斯塔克亚种的伴孢晶体毒素。改进后的程序包括在抗体包被之前用戊二醛对聚苯乙烯比色皿进行预处理。提供了处理过和未处理过的比色皿的直接比较。然后将ELISA用于分析苏云金芽孢杆菌库尔斯塔克亚种和以色列亚种的商业制剂和实验制剂中的杀虫晶体蛋白。
Zotero 插件