用于检测和定量苏云金芽孢杆菌库尔斯塔克亚种和以色列亚种杀虫伴胞晶体蛋白的酶联免疫吸附测定法。
Enzyme-linked immunosorbent assays for detection and quantitation of the entomocidal parasporal crystalline protein of Bacillus thuringiensis subspp. kurstaki and israelensis.
作者信息
Wie S I, Andrews R E, Hammock B D, Faust R M, Bulla L A
出版信息
Appl Environ Microbiol. 1982 Apr;43(4):891-4. doi: 10.1128/aem.43.4.891-894.1982.
Abstract
An enzyme-linked immunosorbent assay was used to detect and quantitate the parasporal crystal toxins of Bacillus thuringiensis subspp. kurstaki and israelensis. The assay method described is extremely sensitive, accurate, and highly specific. With this technique, crystalline insecticidal proteins from several subspecies of B. thuringiensis were compared. The dipteran crystal toxin produced by B. thuringiensis subsp. israelensis was shown to share few epitopes with the lepidopteran toxin from B. thuringiensis subspp. kurstaki, tolworthi, berliner, and alesti.
摘要
采用酶联免疫吸附测定法检测并定量苏云金芽孢杆菌库尔斯塔克亚种和以色列亚种的伴孢晶体毒素。所描述的测定方法极其灵敏、准确且具有高度特异性。利用该技术，对苏云金芽孢杆菌几个亚种的晶体杀虫蛋白进行了比较。结果表明，苏云金芽孢杆菌以色列亚种产生的双翅目晶体毒素与苏云金芽孢杆菌库尔斯塔克亚种、托尔沃思亚种、贝林纳亚种和阿莱斯蒂亚种的鳞翅目毒素几乎没有共同表位。
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