Kohanteb J, Rezai H R, Ardehali S
J Trop Med Hyg. 1984 Oct;87(5):201-5.
Techniques of electroimmunodiffusion (EID) and crossed electroimmunodiffusion (CEID) were applied and compared with the indirect fluorescent antibody (IFA) test for the diagnosis of visceral leishmaniasis. Results obtained by EID on 28 serum samples of proven cases of kala-azar correlated well with IFA. False-positive reactions were encountered by IFA assay among healthy controls and non-kala-azar patients. Using EID technique, species-specific antibody to L. donovani was demonstrated in all sera of kala-azar patients. CEID was less sensitive than EID as a few false-negative results were obtained with this technique. It is concluded that the EID test which is specific, sensitive and more economical could be applied for diagnosis of systemic leishmaniasis.
应用电免疫扩散（EID）和交叉电免疫扩散（CEID）技术，并与间接荧光抗体（IFA）试验进行比较，用于内脏利什曼病的诊断。EID对28例确诊黑热病病例血清样本的检测结果与IFA相关性良好。IFA检测在健康对照和非黑热病患者中出现假阳性反应。使用EID技术，在所有黑热病患者血清中均检测到杜氏利什曼原虫的种特异性抗体。CEID的敏感性低于EID，因为该技术出现了一些假阴性结果。结论是，EID试验具有特异性、敏感性且更经济，可用于系统性利什曼病的诊断。
