In vivo effect to uncoupler CCCP on nitrogenase of Anabaena sp. CH1 and CH2.

Uncoupler CCCP inhibited the growth of Anabaena sp. CH1 and CH2 and reduced their photosynthetic rate and nitrogenase activity. It was determined that upon CCCP addition, nitrogenase activities of CH1 and CH2 declined to almost zero within 1 and 6 hours. Such decline may be caused by the direct inhibition of nitrogenase synthesis rather than by indirect interruption of the phosphorylation process.