疑似临床和实验性广州管圆线虫病的研究：血清学反应。

Studies on suspected clinical and experimental angiostrongyliasis: serological responses.

作者信息

Bhopale M K, Limaye L S, Pradhan V R, Renapurkar D M, Sharma K D

出版信息

J Hyg Epidemiol Microbiol Immunol. 1984;29(3):283-8.

Abstract

Serum antibodies in suspected angiostrongyliasis patient were detected by ELISA. The antibody titre was 1:51,200 in the serum and 1:6,400 in CSF with preadult A. cantonensis antigen. Other tests like AGD and CIEP failed to show any positive reaction with both preadult and adult worm antigens. Experimental infection with 100 A. cantonensis larvae in albino rats indicated positive CIEP reaction in serum from the day 5 to 375 after infection. No precipitin line was seen on the other hand, in AGD during observation period. Different rat groups infected with larval doses of 100, 500, 2,000, and 5,000 showed positive CIEP reaction, on the 21st day of infection when preadult worms were seen in CNS. There was no CIEP reaction when a low dose of 15 larvae was used. Cerebral fluid of rats infected with heavy dose of 5,000 larvae showed positive CIEP reaction on the 21st day.

摘要

采用酶联免疫吸附测定法（ELISA）检测疑似广州管圆线虫病患者的血清抗体。血清中抗体滴度为1:51,200，脑脊液中抗体滴度为1:6,400，采用未成熟广州管圆线虫抗原检测。其他检测方法，如琼脂凝胶扩散试验（AGD）和对流免疫电泳（CIEP），对未成熟和成熟虫体抗原均未显示任何阳性反应。对白化大鼠感染100条广州管圆线虫幼虫的实验表明，感染后第5天至375天血清中CIEP反应呈阳性。另一方面，在观察期内AGD未见沉淀线。感染幼虫剂量分别为100、500、2,000和5,000的不同大鼠组，在感染后第21天，即中枢神经系统中发现未成熟虫体时，CIEP反应呈阳性。当使用15条幼虫的低剂量时，未出现CIEP反应。感染5,000条幼虫高剂量的大鼠脑脊液在第21天CIEP反应呈阳性。