de Leeuw P W, de Bos R, van Es P N, Birkenhäger W H
J Hypertens Suppl. 1984 Dec;2(3):S225-6.
To investigate whether human blood platelets contain angiotensin II, platelets were isolated from platelet-rich plasma and resuspended in buffer solution. No angiotensin was detectable by radio-immunoassay in this suspension. However, after sonification all samples appeared to contain significant amounts of angiotensin II. This amount was not reduced by the addition of angiotensinase inhibitors, thus ruling out an assay artefact due to breakdown of tracer. Subsequent experiments showed that the immunoreactive material behaved similarly to standard angiotensin II in several respects.
为了研究人类血小板中是否含有血管紧张素II，从富含血小板的血浆中分离出血小板，并将其重悬于缓冲溶液中。通过放射免疫分析在该悬浮液中未检测到血管紧张素。然而，超声处理后，所有样品似乎都含有大量的血管紧张素II。添加血管紧张素酶抑制剂后，该量并未减少，因此排除了由于示踪剂分解导致的检测假象。随后的实验表明，免疫反应性物质在几个方面的表现与标准血管紧张素II相似。
