Büchel D E, Gronenborn B, Müller-Hill B
Nature. 1980 Feb 7;283(5747):541-5. doi: 10.1038/283541a0.
The nucleotide sequence of the lacY gene coding for lactose permease (M protein) in Escherichia coli has been determined. The sequence includes the intergenic regions between the lacZ (beta-galactosidase) and lacY genes as well as the region between the lacY and lacA (transacetylase) genes. Lactose permease is predicted to consist of 417 residues (71% nonpolar), resulting in a protein with a molecular weight of 46,504. The reading frame was confirmed by the sequence of a nonsense mutation changing codon 33 from UGG to UAG.
已确定大肠杆菌中编码乳糖通透酶（M蛋白）的lacY基因的核苷酸序列。该序列包括lacZ（β-半乳糖苷酶）和lacY基因之间的基因间隔区以及lacY和lacA（转乙酰酶）基因之间的区域。预测乳糖通透酶由417个残基组成（71%为非极性），产生一种分子量为46,504的蛋白质。通过将密码子33从UGG改变为UAG的无义突变序列证实了阅读框。
