Ehring R, Beyreuther K, Wright J K, Overath P
Nature. 1980 Feb 7;283(5747):537-40. doi: 10.1038/283537a0.
The lacY gene product synthesised in vitro is identical to lactose permease isolated from cytoplasmic membranes as determined by apparent molecular weight and N-terminal amino acid sequence. The amino acid composition of the in vivo product agrees well with that predicted from the DNA sequence. The data assign the translational start on the DNA sequence and demonstrate that this protein is processed only by deformylation but not by proteolytic cleavage at the N-terminus.
体外合成的lacY基因产物与从细胞质膜分离的乳糖通透酶在表观分子量和N端氨基酸序列方面相同。体内产物的氨基酸组成与根据DNA序列预测的结果非常吻合。这些数据确定了DNA序列上的翻译起始位点，并表明该蛋白质仅通过去甲酰化进行加工，而在N端不进行蛋白水解切割。
