Kastner M, Maurer H R
Hoppe Seylers Z Physiol Chem. 1980;361(2):197-200.
In order to isolate an endogenous inhibitor (chalone) of granulopoiesis, a source of chalone has been employed which consists of pure granulocytes. Optimal conditions for the isolation of these cells from bovine blood using EDTA are described. After incubation of the cells, the conditioned medium was lyophilised and extracted in two separate procedures with water or acetone. Sephadex G-10 chromatography yielded a fraction having a molecular weight under 700 which was capable of inhibiting the colony formation of myelopoietic stem cells in a nontoxic, cell-specific and reversible manner. THE INHIBITORy activity of the fraction was found to be dependent on the addition of 2-mercaptoethanol. Even then, aqueous solutions of the inhibitor are at present not indefinitely stable.
为了分离粒细胞生成的内源性抑制剂（抑素），采用了由纯粒细胞组成的抑素来源。描述了使用乙二胺四乙酸（EDTA）从牛血中分离这些细胞的最佳条件。细胞孵育后，将条件培养基冻干，并分别用水或丙酮进行两步提取。葡聚糖G - 10柱层析得到一个分子量低于700的组分，该组分能够以无毒、细胞特异性和可逆的方式抑制骨髓造血干细胞的集落形成。发现该组分的抑制活性依赖于添加2 - 巯基乙醇。即便如此，目前该抑制剂的水溶液仍不能无限期稳定保存。
