Privat de Garilhe M, Vedel M, Tempête C, Federici C, Garcia-Giralt E
Biomedicine. 1980 Oct;32(3):148-54.
Lymphoid chalone extracts, obtained from bovine spleen were purified on chromatographic columns: in the first step of the procedure, exclusion chromatography on Sephadex G75, and ion exchange chromatography on DEAE Sephadex were applied. A purified, active material was thus obtained. Upon thin layer chromatography, this fraction was shown to contain 3-4 UV absorbing components and 2 ninhydrin positive components. Further purification on Biogel P2, on Sephadex G10 and preparative thin layer chromatography, showed that the biological activity was located in small molecular weight components which belong to the polypeptide series. The ultraviolet absorbing components were identified as known nucleosides. The purified material shows an inhibitory effect on the uptake of 3H-thymidine by lymphocytes, as well as on mitogen induced lymphocyte transformation and haemolysin plaque forming cells. Furthermore, this inhibitory effect is specific for lymphoid cells.
在该程序的第一步，应用了Sephadex G75凝胶过滤色谱和DEAE Sephadex离子交换色谱。由此获得了一种纯化的活性物质。经薄层色谱分析，该级分显示含有3 - 4个吸收紫外线的成分和2个茚三酮阳性成分。在Biogel P2、Sephadex G10上进一步纯化以及制备薄层色谱表明，生物活性位于属于多肽系列的小分子成分中。吸收紫外线的成分被鉴定为已知的核苷。纯化后的物质对淋巴细胞摄取3H - 胸腺嘧啶核苷、对丝裂原诱导的淋巴细胞转化以及溶血素空斑形成细胞均有抑制作用。此外，这种抑制作用对淋巴细胞具有特异性。
