Reinherz E L, Kung P C, Goldstein G, Schlossman S F
J Immunol. 1980 Mar;124(3):1301-7.
A hybridoma-secreting monoclonal antibody was produced from the spleen cells of a mouse immunized with human thymocytes. This hybridoma antibody, termed OKT5, was reactive by indirect immunofluorescence with 80% of human thymocytes but only 20% of peripheral blood T cells. Moreover, OKT5 was unreactive with normal B cells, null cells, and macrophages at any dilution tested. A similar pattern of reactivity was seen with an equine antiserum to human thymocytes termed anti-TH2. Fluorescence-activated cell sorting demonstrated that the OKT5 antibody reactivity on peripheral T cells was restricted to the majority of the previously defined TH2+ subpopulation. In functional studies, the OKT5+ subset, like the TH2+ subset, proliferated well to the mitogen Con A and to alloantigens, and contained cytotoxic effector cells after sensitization in MLC, and suppressor effector cells after activation with Con A. In addition, like the TH2+ T cell, the OKT+ T cell was virtually unresponsive to soluble antigen. Thus, the OKT5 monoclonal antibody is reactive with the cytotoxic/suppressor T cell subset. OKT5 should provide an important probe to assess the status of suppressor cells in human disease.
用人类胸腺细胞免疫小鼠，从小鼠脾脏细胞中制备出一种分泌单克隆抗体的杂交瘤。这种杂交瘤抗体称为OKT5，通过间接免疫荧光法可与80%的人类胸腺细胞发生反应，但仅与20%的外周血T细胞发生反应。此外，在任何测试稀释度下，OKT5与正常B细胞、裸细胞和巨噬细胞均无反应。用一种针对人类胸腺细胞的马抗血清（称为抗TH2）也观察到了类似的反应模式。荧光激活细胞分选显示，OKT5抗体在外周T细胞上的反应性仅限于大多数先前定义的TH2+亚群。在功能研究中，OKT5+亚群与TH2+亚群一样，对有丝分裂原刀豆球蛋白A和同种异体抗原增殖良好，在混合淋巴细胞培养中致敏后含有细胞毒性效应细胞，用刀豆球蛋白A激活后含有抑制效应细胞。此外，与TH2+T细胞一样，OKT+T细胞对可溶性抗原几乎无反应。因此，OKT5单克隆抗体与细胞毒性/抑制性T细胞亚群发生反应。OKT5应提供一个重要的探针来评估人类疾病中抑制细胞的状态。
