Wu S, Bach F H, Sopori M
J Immunol. 1980 May;124(5):2464-7.
Thymocytes used as responding cells in a mixed leukocyte culture with x-irradiated splenic stimulating cells generate highly significant proliferative and cytotoxic responses when responding and stimulating cells differ by the entire H-2 complex. On the other hand, when the genetic difference between responding and stimulating cells is only a K region, very little, if any, proliferative response is detectable and no cytotoxic response is found. In contrast, when responding and stimulating cell donors differ by a spontaneous mutation in the K region of the H-2 complex, as found in B6.C-H-2ba, B6-H-2bd and B6.C-H-2bf, highly significant proliferative and cytotoxic responses can be obtained. These results, thus, argue that the H-2 mutants cannot, with regard to their relationship to the parental strain, be readily equated with a K region difference as defined in the recombinant inbred strains.
在用经X射线照射的脾刺激细胞进行的混合淋巴细胞培养中，当反应细胞和刺激细胞在整个H-2复合体上存在差异时，用作反应细胞的胸腺细胞会产生高度显著的增殖和细胞毒性反应。另一方面，当反应细胞和刺激细胞之间的遗传差异仅为一个K区域时，几乎检测不到增殖反应，也未发现细胞毒性反应。相比之下，当反应细胞供体和刺激细胞供体在H-2复合体的K区域存在自发突变时，如在B6.C-H-2ba、B6-H-2bd和B6.C-H-2bf中发现的那样，可以获得高度显著的增殖和细胞毒性反应。因此，这些结果表明，就其与亲本菌株的关系而言，H-2突变体不能轻易等同于重组近交系中定义的K区域差异。
