Wu S, Bach F H
J Immunol. 1981 Feb;126(2):775-80.
Thymocytes used as responding cells in a mixed leukocyte culture with gamma-irradiated splenic stimulating cells generated highly significant proliferative and cytolytic responses when responding and stimulating cells differed by the entire H-2 complex or at H-2K plus H-21 regions. On the other hand, when the difference was only an H-2K region, very little, if any, proliferative response was detectable and no cytolytic response was found. Because it has been claimed by others that thymocytes do not include cells needed to provide the help required for the generation of a cytolytic response and yet we have found a highly significant response in thymocytes, we have analyzed, in 2 systems, the possible role of back-response by stimulating spleen cells in the generation of cytolytic activity by responding thymocytes. In the first system, spleen cells were depleted of T lymphocytes by treatment with anti-Thy-1.2 antiserum plus complement. In the second system, F1 hybrid mouse spleen cells were used to stimulate parental responding thymocytes. In both cases, no back-response as measured by cell proliferation in mixed leukocyte culture was detected, while both types of spleen cells still stimulate significant cell proliferation and cytolytic activity from responding thymocytes, as well as from responding spleen cells. Furthermore, it was found that in the presence of stimulating spleen cells (either T lymphocyte-depleted or not) that differ from the responding thymocytes at the entire H-2 complex, thymocytes were capable of generating a significant and specific cytolytic response to H-2K region different stimulating spleen cells (either T lymphocyte-depleted or not). These results are compatible with the suggestion that there are sufficient numbers of helper cells in the unfractionated thymocyte population for the generation of primary cytolytic T lymphocyte responses in vitro.
在与经γ射线照射的脾刺激细胞进行的混合淋巴细胞培养中，当反应细胞（胸腺细胞）和刺激细胞在整个H-2复合体或H-2K加H-2I区域存在差异时，用作反应细胞的胸腺细胞会产生高度显著的增殖反应和细胞溶解反应。另一方面，当差异仅存在于H-2K区域时，几乎检测不到增殖反应（如果有增殖反应的话），并且未发现细胞溶解反应。因为其他人声称胸腺细胞不包含产生细胞溶解反应所需的辅助细胞，然而我们在胸腺细胞中发现了高度显著的反应，所以我们在两个系统中分析了刺激脾细胞的反向反应在反应性胸腺细胞产生细胞溶解活性过程中的可能作用。在第一个系统中，用抗Thy-1.2抗血清加补体处理脾细胞以去除T淋巴细胞。在第二个系统中，使用F1杂种小鼠脾细胞刺激亲代反应性胸腺细胞。在这两种情况下，通过混合淋巴细胞培养中的细胞增殖测量，均未检测到反向反应，而两种类型的脾细胞仍能刺激反应性胸腺细胞以及反应性脾细胞产生显著的细胞增殖和细胞溶解活性。此外，还发现，在整个H-2复合体与反应性胸腺细胞不同的刺激脾细胞（无论是否去除T淋巴细胞）存在的情况下，胸腺细胞能够对H-2K区域不同的刺激脾细胞（无论是否去除T淋巴细胞）产生显著且特异性的细胞溶解反应。这些结果与以下观点相符，即未分级的胸腺细胞群体中有足够数量的辅助细胞用于在体外产生原发性细胞溶解T淋巴细胞反应。
