Argyris B F
J Natl Cancer Inst. 1980 Apr;64(4):959-66.
Adult C57BL/6 mice inoculated with P-815 mastocytoma cells developed splenic suppressor cells. These suppressor cells were assayed in mixed lymphocyte cultures. Suppressor cells appeared earlier and became more active after sensitization with low doses of allogeneic tumor. After low doses of tumor cells, the suppressor cell activity developed before cytotoxic activity, whereas after higher doses of tumor cells, suppressor and cytotoxic activity appeared simultaneously. The suppressor cells were not H-2 restricted but did express their activity stronger in the presence of the specific (H-2d) alloantigen. Neonatally thymectomized adult C57BL/6 mice failed to develop splenic suppressor cell activity after tumor allosensitization. This suggested that the thymus is a source for suppressor precursor cells. These precursor cells remained active in the spleen up to 3 1/2 months after adult thymectomy. Suppressor factor was released in cultures of tumor-allosensitized spleen cells. The presence of a specific (H-2d) alloantigen improved the relase of suppressor factor (SF). Cultures of tumor-allosensitized spleen cells, enriched for T-cells, contained more SF. SF was not H-2 restricted or antigen-specific.
接种P - 815肥大细胞瘤细胞的成年C57BL/6小鼠产生了脾抑制细胞。这些抑制细胞在混合淋巴细胞培养物中进行检测。在用低剂量同种异体肿瘤致敏后，抑制细胞出现得更早且变得更活跃。在低剂量肿瘤细胞接种后，抑制细胞活性在细胞毒性活性之前出现，而在高剂量肿瘤细胞接种后，抑制细胞和细胞毒性活性同时出现。抑制细胞不受H - 2限制，但在特异性（H - 2d）同种异体抗原存在时其活性表达更强。新生期胸腺切除的成年C57BL/6小鼠在肿瘤同种致敏后未能产生脾抑制细胞活性。这表明胸腺是抑制前体细胞的来源。这些前体细胞在成年胸腺切除后在脾脏中保持活性长达3个半月。在肿瘤同种致敏的脾细胞培养物中释放出抑制因子。特异性（H - 2d）同种异体抗原的存在促进了抑制因子（SF）的释放。富含T细胞的肿瘤同种致敏脾细胞培养物含有更多的SF。SF不受H - 2限制，也不是抗原特异性的。
