Argyris B F
Cancer Res. 1977 Sep;37(9):3390-9.
C57BL/6 mice were sensitized i.p. with 20 X 10(6) P-815 mastocytoma cells. Spleen and lymph node cells from these tumor-allosensitized mice become hyporesponsive in mixed-lymphocyte culture. The hyporesponsiveness appears to be due to the presence of suppressor cells. These suppressor cells can be demonstrated by their ability to inhibit the mixed-lymphocyte culture reactivity of normal mouse spleen cells. Suppressor activity in the spleen of tumor-allosensitized mice remains long after cytotoxic activity disappears. The mixed-lymphocyte culture suppressor cells do not adhere to glass and are cortisone resistant, X-irradiation resistant, anti-immunoglobulin serum resistant, but are anti-theta (Thy 1.2) serum sensitive. Adsorption of tumor-allosensitized spleen on specific allogeneic monolayers removes cytotoxic but not suppressor cells. On the basis of the kinetic data, cortisone sensitivity, antisera sensitivity, and adsorption results, we conclude that the suppressor cell is a thymus-derived lymphocyte that is separate from the cytotoxic thymus-derived lymphocyte.
将20×10⁶个P - 815肥大细胞瘤细胞腹腔注射使C57BL/6小鼠致敏。来自这些肿瘤同种致敏小鼠的脾细胞和淋巴结细胞在混合淋巴细胞培养中变得反应低下。这种反应低下似乎是由于抑制细胞的存在。这些抑制细胞可通过其抑制正常小鼠脾细胞混合淋巴细胞培养反应性的能力得以证实。在细胞毒性活性消失后很长时间，肿瘤同种致敏小鼠脾中的抑制活性仍然存在。混合淋巴细胞培养抑制细胞不黏附于玻璃，对可的松有抗性，对X射线有抗性，对抗免疫球蛋白血清有抗性，但对抗θ（Thy 1.2）血清敏感。将肿瘤同种致敏脾吸附于特异性同种异基因单层上可去除细胞毒性细胞，但不能去除抑制细胞。根据动力学数据、可的松敏感性、抗血清敏感性和吸附结果，我们得出结论，抑制细胞是一种源自胸腺的淋巴细胞，它与细胞毒性源自胸腺的淋巴细胞不同。
