Chen H F, Nakabayashi M, Satoh K, Sakamoto S
Thromb Haemost. 1980 Feb 29;42(5):1536-47.
A new method is described for the preparation of highly purified human plasminogen and plasmin with specific activity of 32 CTA units per mg of protein. With this method, the purification of the urinary plasminogen + plasmin antigenic materials from patients with chronic glomerulonephritis, disseminated intravascular coagulation syndrome and severe toxemia of pregnancy was performed, and the resulting highly purified proenzyme and enzyme were analyzed by immunoelectrophoresis, separative agar electrophoresis, gel filtration and SDS-gel electrophoresis. Our findings indicated that urinary plasmin reflects more closely the extent of intraglomerular fibrinolysis, while urinary plasminogen reflects non-selective proteinuria in patients with chronic glomerulonephritis or severe toxemia of pregnancy.
本文描述了一种制备高纯度人纤溶酶原和纤溶酶的新方法，其比活性为每毫克蛋白质32个CTA单位。采用该方法，对慢性肾小球肾炎、弥散性血管内凝血综合征和重度妊娠毒血症患者尿液中的纤溶酶原+纤溶酶抗原物质进行了纯化，并通过免疫电泳、琼脂糖分离电泳、凝胶过滤和SDS凝胶电泳对所得的高纯度酶原和酶进行了分析。我们的研究结果表明，尿液中的纤溶酶更能准确反映肾小球内纤维蛋白溶解的程度，而尿液中的纤溶酶原则反映了慢性肾小球肾炎或重度妊娠毒血症患者的非选择性蛋白尿。
