Confer A W, Adldinger H K
Am J Vet Res. 1980 Mar;41(3):307-12.
Two experiments were conducted to study the cell-mediated cytotoxicity of peripheral blood leukocytes (PBL) from chickens inoculated with Marek's disease virus (MDV) against a Marek's disease-derived lymphoblastoid cell line (MSB-1) and to associate the cytotoxicity with incidence of disease. In experiment I, moderately susceptible random-bred, specific-pathogen-free chickens were inoculated with MDV (group 1), vaccinated with a herpesvirus of turkeys (HVT) and inoculated with MDV (group 2), vaccinated with HVT and inoculated with chicken kidney cells (CKC; group 3), and inoculated with CKC only (group 4). Cytotoxic activity in the PBL was detected initially during the first week after MDV inoculation and periodically throughout the observation period (groups 1, 2, and 3). Throughout the observation period, the magnitude of cytotoxic activity was similar in PBL from groups 1 and 2 chickens. The PBL from both surviving and fatally infected chickens (groups 1 and 2) were similarly cytotoxic when sampled during the first 16 days after MDV inoculation. In experiment II, inbred genetically susceptible (line 7) and resistant (line 6) chickens were used. Cytotoxic activity of PBL of significantly greater magnitude was associated with a lower mortality or incidence of gross lesions (or both) in MDV-inoculated line 6 (group B) and HVT-vaccinated and MDV-inoculated line 7 (group C) chickens compared with activity of PBL from MDV-inoculated line 7 (group A) chickens. The cytotoxic activity of PBL from individual inbred chickens did not correlate with the outcome of the infection.
进行了两项实验，以研究接种马立克氏病病毒（MDV）的鸡外周血白细胞（PBL）对源自马立克氏病的淋巴母细胞系（MSB-1）的细胞介导细胞毒性，并将细胞毒性与疾病发病率相关联。在实验I中，将中度易感的随机繁殖、无特定病原体的鸡接种MDV（第1组），用火鸡疱疹病毒（HVT）疫苗接种并接种MDV（第2组），用HVT疫苗接种并接种鸡肾细胞（CKC；第3组），以及仅接种CKC（第4组）。在接种MDV后的第一周最初检测到PBL中的细胞毒性活性，并在整个观察期内定期检测（第1、2和3组）。在整个观察期内，第1组和第2组鸡的PBL中细胞毒性活性的大小相似。在接种MDV后的前16天取样时，存活和致命感染鸡（第1组和第2组）的PBL具有相似的细胞毒性。在实验II中，使用了近交系遗传易感（7系）和抗性（6系）鸡。与接种MDV的7系（A组）鸡的PBL活性相比，接种MDV的6系（B组）和接种HVT并接种MDV的7系（C组）鸡中，PBL的细胞毒性活性显著更高，与较低的死亡率或大体病变发生率（或两者）相关。个体近交系鸡的PBL细胞毒性活性与感染结果无关。
