Han T
Cancer. 1980 Apr 15;45(8):2102-8. doi: 10.1002/1097-0142(19800415)45:8<2102::aid-cncr2820450818>3.0.co;2-i.
The present study was undertaken to elucidate the type and the role of suppressor cells on T lymphocyte response to PHA in untreated and treated patients with Hodgkin's disease. The mean value of peripheral blood T lymphocyte response to PHA in untreated or treated patients was lower than that in healthy subjects. However, the difference was not statistically significant. There was no significant difference in T lymphocyte response between localized and generalized stage or between different cell types in both active and remission Hodgkin's disease patients. The mean T lymphocyte response to PHA of active patients without systemic symptoms (A), on the other hand, was significantly higher than that of active patients with systemic symptoms (B). The mean value of T lymphocyte response in the presence of monocyte-enriched E- cells was significantly lower, whereas the mean value in the presence of monocyte-depleted E- cells was only moderately lower than that in the absence of these non-T cells in active patients. The mean value of PHA response of T lymphocytes with monocyte-enriched or monocyte-depleted E- cells was only slightly lower than PHA response without these cells in remission patients. No suppressor cell activity of T cells was observed in both active and remission patients. The only significant difference in the suppressor cell activity of monocyte-enriched E- cells on the T lymphocyte response to PHA was observed between localized and generalized stage in patients with active disease. Unlike peripheral blood monocyte-enriched E- cells, the splenic monocyte-enriched E- cells possessed no significant suppressor cell activity on the splenic T lymphocyte response to PHA in active Hodgkin's disease patients. These observations suggest that the depression of in vitro cell-mediated immunity seen in patients with active Hodgkin's disease may be due to the non-specific suppression of T lymphocyte response by monocytes, in addition to a possibly intrinsic defect of T lymphocytes.
本研究旨在阐明霍奇金病未经治疗和经治疗患者中抑制细胞对T淋巴细胞对PHA反应的类型和作用。未经治疗或经治疗患者外周血T淋巴细胞对PHA的反应平均值低于健康受试者。然而，差异无统计学意义。在活动期和缓解期的霍奇金病患者中，局限性和全身性分期之间或不同细胞类型之间的T淋巴细胞反应无显著差异。另一方面，无全身症状的活动期患者（A）对PHA的平均T淋巴细胞反应显著高于有全身症状的活动期患者（B）。在活动期患者中，存在富含单核细胞的E细胞时T淋巴细胞反应的平均值显著降低，而存在去除单核细胞的E细胞时平均值仅略低于不存在这些非T细胞时。在缓解期患者中，有或无富含单核细胞或去除单核细胞的E细胞时T淋巴细胞的PHA反应平均值仅略低于无这些细胞时。在活动期和缓解期患者中均未观察到T细胞的抑制细胞活性。在活动期疾病患者中，仅在局限性和全身性分期之间观察到富含单核细胞的E细胞对T淋巴细胞对PHA反应的抑制细胞活性存在显著差异。与外周血富含单核细胞的E细胞不同，在活动期霍奇金病患者中，脾脏富含单核细胞的E细胞对脾脏T淋巴细胞对PHA的反应不具有显著的抑制细胞活性。这些观察结果表明，活动期霍奇金病患者中所见的体外细胞介导免疫抑制可能是由于单核细胞对T淋巴细胞反应的非特异性抑制，此外还可能存在T淋巴细胞的内在缺陷。
