Ashman C R, Davidson R L
J Cell Physiol. 1980 Jan;102(1):45-50. doi: 10.1002/jcp.1041020107.
These studies were undertaken to examine the relationship between the inhibition by 5-bromodeoxyuridine (BrdU) of erythroid differentiation in Friend erythroleukemia cells and the incorporation of BrdU into DNA. Experiments were carried out in which the incorporation of BrdU into DNA and the concentration of BrdU to which the cells were exposed were varied independently of each other. In addition, the ability of deoxycytidine (dC) to reverse the effects of BrdU on hemoglobin production and to reduce the amount of BrdU in DNA was analyzed. Under all the conditions tested, the effects of BrdU were correlated with the amount of BrdU incorporated into nuclear DNA. These results differ from those of recent studies on the inhibition of pigmentation and the induction of mutations by BrdU in Syrian hamster melanoma cells. The results suggest that BrdU may be producing its biological effects by a variety of different mechanisms.
开展这些研究是为了检验5-溴脱氧尿苷（BrdU）对Friend红白血病细胞红系分化的抑制作用与BrdU掺入DNA之间的关系。进行了相关实验，其中BrdU掺入DNA的情况以及细胞所接触的BrdU浓度彼此独立变化。此外，还分析了脱氧胞苷（dC）逆转BrdU对血红蛋白产生的影响以及减少DNA中BrdU含量的能力。在所有测试条件下，BrdU的作用都与掺入核DNA的BrdU量相关。这些结果与最近关于BrdU对叙利亚仓鼠黑色素瘤细胞色素沉着的抑制作用和诱导突变的研究结果不同。结果表明，BrdU可能通过多种不同机制产生其生物学效应。
