[Radioimmunoassay of dehydroepiandrosterone and 5-androsten-3 beta, 17 beta-diol (author's transl)].

A radioimmunological method is described for the determination of dehydroepiandrosterone and 5-androsten-3 beta, 17 beta-diol in plasma. Interfering cross-reactions of the antiserum of 5-androsten-3 beta, 17 beta-diol with testosterone and pregnenolone make it necessary to separate the steroids by thin-layer chromatography before their final determination. With respect to sensitivity, this method is superior to the commonly used photometric and gas-chromatographic analytical procedures. For both steroids the limits of detection are about 170 fmol.