Benes P, Morsches B, Holzmann H
J Clin Chem Clin Biochem. 1980 Feb;18(2):117-22.
A radioimmunological method is described for the determination of dehydroepiandrosterone and 5-androsten-3 beta, 17 beta-diol in plasma. Interfering cross-reactions of the antiserum of 5-androsten-3 beta, 17 beta-diol with testosterone and pregnenolone make it necessary to separate the steroids by thin-layer chromatography before their final determination. With respect to sensitivity, this method is superior to the commonly used photometric and gas-chromatographic analytical procedures. For both steroids the limits of detection are about 170 fmol.
本文描述了一种用于测定血浆中脱氢表雄酮和5-雄烯-3β,17β-二醇的放射免疫分析方法。5-雄烯-3β,17β-二醇抗血清与睾酮和孕烯醇酮之间存在干扰性交叉反应，因此在最终测定之前，有必要通过薄层色谱法分离这些类固醇。在灵敏度方面，该方法优于常用的光度分析和气相色谱分析方法。对于这两种类固醇，检测限约为170飞摩尔。
