Forti G, Calabresi E, Giannotti P, Borrelli D, Gonnelli P, Barbieri U, Serio M
Horm Res. 1978;9(4):194-200. doi: 10.1159/000178913.
An original method for 5-androsten-3beta,17beta-diol (A-diol) measurement using an antiserum against A-diol-16-CMO-BSA is described. A-diol and testosterone (T) were determined by radioimmunoassay methods in spermatic and peripheral venous plasma of nine normal subjects during surgical intervention for inguinal hernia repair. In spermatic venous plasma the levels of T and A-diol were, respectively, 25.9 +/- 13.3 and 4.8 +/- 5.1 microgram/100ml (mean +/- SD) with an A-diol/T ratio of 0.19 +/- 0.15 (mean +/- SD); in peripheral plasma the levels of T and A-diol were, respectively, 269 +/- 58 and 91 +/- 25 ng/100 ml (mean +/- SD) with an A-diol/T ratio of 0.35 +/- 0.12 (mean +/- SD) significantly different from spermatic venous plasma (p less than 0.01). From these data a mean testicular A-diol secretion of about 0.70 mg/24 h can be calculated: this value corresponds approximately to the 50% of the blood production rate (BPR) of this steroid. So it can be assumed that a large amount of A-diol in systemic blood comes from sources outside the male gonad.
描述了一种使用抗A-二醇-16-CMO-BSA抗血清测量5-雄烯-3β,17β-二醇（A-二醇）的原始方法。在腹股沟疝修补手术干预期间，通过放射免疫分析法测定了9名正常受试者的精索静脉血浆和外周静脉血浆中的A-二醇和睾酮（T）。在精索静脉血浆中，T和A-二醇的水平分别为25.9±13.3和4.8±5.1微克/100毫升（平均值±标准差），A-二醇/T比值为0.19±0.15（平均值±标准差）；在外周血浆中，T和A-二醇的水平分别为269±58和91±25纳克/100毫升（平均值±标准差），A-二醇/T比值为0.35±0.12（平均值±标准差），与精索静脉血浆有显著差异（p<0.01）。根据这些数据，可以计算出睾丸A-二醇的平均分泌量约为0.70毫克/24小时：该值约相当于该类固醇血生成率（BPR）的50%。因此，可以假设全身血液中的大量A-二醇来自男性性腺以外的来源。
